Bioactive Peptides from Tempeh Using Peptidecutter’s Cleavage

نویسندگان

چکیده

empeh is an Indonesian traditional fermented food with rich nutrition and bioactive components. Rhizopus sp,especially oligosporus), lactic acid bacteria (Lactobacillus sp.), yeast aremicroorganisms involved in Tempeh fermentation. An interesting offer of Bioinformatics (in silico method) as a supporting tool molecular biology studies has emerged, such protein cleavage. This study utilized PeptideCutter application on ExPASyBioinformatics portal (https://web.expasy.org/peptide_cutter/) to cleave soy proteins glycinin G1, G2, G3, G4,G5,?-conglycinin-??chain,and??chainusingavailable enzymes the applicationwith twosimulations. Simulation I was conducted using enzyme complex produced by Lactobacillus sp.and oligosporus, whilesimulation II used sp., Klebsiella pneumonia. sp. pneumoniae. A total 58 peptides found from simulation higher than (41 peptides). The cleavages PeptideCuttertool were dominated dipeptides only three form tripeptides, namely Leu-Leu-Phe(glycininG1), Val-Val-Phe(glycininG5), Arg-His-Lys(?-conglycinin-??chain). Bioactive antihypertensive antidiabetic properties mostly this silicomethod soybean

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ژورنال

عنوان ژورنال: Bioinformatics and biomedical research journal

سال: 2021

ISSN: ['2620-3324']

DOI: https://doi.org/10.11594/bbrj.03.02.05